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pgex 5x3 vector  (Danaher Inc)


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    Structured Review

    Danaher Inc pgex 5x3 vector
    Pgex 5x3 Vector, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+pgex+5x+3/PGEX-5X-3/pmc02833144-209-11-13
    Average 95 stars, based on 304 article reviews
    pgex 5x3 vector - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cloning:

    Article Title: MNNG-induced Cell Death Is Controlled by Interactions between PARP-1, Poly(ADP-ribose) Glycohydrolase, and XRCC1
    Article Snippet: .. Cloning and Purification of Proteins—The cDNA encoding amino acids 378–976 of human PARG (accession number DQ867088) was cloned as a SalI fragment into the vector pGEX-5X-3 (Amersham Biosciences). .. Expression of glutathione S-transferase (GST) or the GST-PARG65 construct was performed in Escherichia coli BL21 Codon Plus-RIL cells (Stratagene), which additionally were transfected with the pREP4 plasmid (Qiagen).

    Purification:

    Article Title: MNNG-induced Cell Death Is Controlled by Interactions between PARP-1, Poly(ADP-ribose) Glycohydrolase, and XRCC1
    Article Snippet: .. Cloning and Purification of Proteins—The cDNA encoding amino acids 378–976 of human PARG (accession number DQ867088) was cloned as a SalI fragment into the vector pGEX-5X-3 (Amersham Biosciences). .. Expression of glutathione S-transferase (GST) or the GST-PARG65 construct was performed in Escherichia coli BL21 Codon Plus-RIL cells (Stratagene), which additionally were transfected with the pREP4 plasmid (Qiagen).

    Clone Assay:

    Article Title: MNNG-induced Cell Death Is Controlled by Interactions between PARP-1, Poly(ADP-ribose) Glycohydrolase, and XRCC1
    Article Snippet: .. Cloning and Purification of Proteins—The cDNA encoding amino acids 378–976 of human PARG (accession number DQ867088) was cloned as a SalI fragment into the vector pGEX-5X-3 (Amersham Biosciences). .. Expression of glutathione S-transferase (GST) or the GST-PARG65 construct was performed in Escherichia coli BL21 Codon Plus-RIL cells (Stratagene), which additionally were transfected with the pREP4 plasmid (Qiagen).

    Article Title: Immune response in Helicobacter pylori-induced low-grade gastric-mucosa-associated lymphoid tissue (MALT) lymphoma.
    Article Snippet: The PCR was performed in a 20 l volume containing ExTaq polymerase (Takara), 5 mM sense and anti-sense oligonucleotide primers and 500 ng H. pylori genomic DNA. .. The cycling conditions were 25 22 Journal of Medical Microbiology 53 cycles of 94 8C for 30 s, 55 8C for 30 s and 72 8C for 30 s. The amplicon was cut with BamHI and SalI restriction endonucleases and cloned into the vector pGEX-5X-3 (Amersham Pharmacia) by standard techniques; the resultant plasmid was transformed into Escherichia coli DH-5Æ. ..

    Amplification:

    Article Title: Immune response in Helicobacter pylori-induced low-grade gastric-mucosa-associated lymphoid tissue (MALT) lymphoma.
    Article Snippet: The PCR was performed in a 20 l volume containing ExTaq polymerase (Takara), 5 mM sense and anti-sense oligonucleotide primers and 500 ng H. pylori genomic DNA. .. The cycling conditions were 25 22 Journal of Medical Microbiology 53 cycles of 94 8C for 30 s, 55 8C for 30 s and 72 8C for 30 s. The amplicon was cut with BamHI and SalI restriction endonucleases and cloned into the vector pGEX-5X-3 (Amersham Pharmacia) by standard techniques; the resultant plasmid was transformed into Escherichia coli DH-5Æ. ..

    Plasmid Preparation:

    Article Title: Immune response in Helicobacter pylori-induced low-grade gastric-mucosa-associated lymphoid tissue (MALT) lymphoma.
    Article Snippet: The PCR was performed in a 20 l volume containing ExTaq polymerase (Takara), 5 mM sense and anti-sense oligonucleotide primers and 500 ng H. pylori genomic DNA. .. The cycling conditions were 25 22 Journal of Medical Microbiology 53 cycles of 94 8C for 30 s, 55 8C for 30 s and 72 8C for 30 s. The amplicon was cut with BamHI and SalI restriction endonucleases and cloned into the vector pGEX-5X-3 (Amersham Pharmacia) by standard techniques; the resultant plasmid was transformed into Escherichia coli DH-5Æ. ..

    Transformation Assay:

    Article Title: Immune response in Helicobacter pylori-induced low-grade gastric-mucosa-associated lymphoid tissue (MALT) lymphoma.
    Article Snippet: The PCR was performed in a 20 l volume containing ExTaq polymerase (Takara), 5 mM sense and anti-sense oligonucleotide primers and 500 ng H. pylori genomic DNA. .. The cycling conditions were 25 22 Journal of Medical Microbiology 53 cycles of 94 8C for 30 s, 55 8C for 30 s and 72 8C for 30 s. The amplicon was cut with BamHI and SalI restriction endonucleases and cloned into the vector pGEX-5X-3 (Amersham Pharmacia) by standard techniques; the resultant plasmid was transformed into Escherichia coli DH-5Æ. ..



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